tnf α recombinant antibody  (Proteintech)

Journal: Bioactive Materials

Article Title: Mossy-textured hydroxyapatite-modified poly (lactic-co-glycolic acid) microspheres promote collagen regeneration via calcium/TGF-β and chemokine signaling pathways in soft tissue augmentation

doi: 10.1016/j.bioactmat.2025.12.028

Figure Lengend Snippet: The regulatory effects of CaHA/PLGA microspheres on macrophages and ADSCs in vitro. (A, B) CLSM images and RFI of CD86 and CD206 expression in RAW264.7 cells co-cultured with microspheres for 2 days (n = 3). (C–F) Relative mRNA expression levels of inflammation-related genes TNF-α, IL-6, TGF-β1, and FGF-2 in RAW264.7 cells (n = 3). (G, H) Sirius red staining images and quantitative analysis (n = 3) of collagen deposition of ADSCs co-cultured with CaHA/PLGA microspheres for 3 and 7 days. (I–K) Relative mRNA expression levels of TGF-β1, FGF-2, and PDGF-A in ADSCs after 3 and 7 days of co-culture with CaHA/PLGA microspheres (n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.

Article Snippet: CD86 polyclonal antibody (1:200, 13395-1-AP, Proteintech), CD206 (1:200, 18704-1-AP, Proteintech), and TNF-α recombinant antibody (1:200, 80258-6-RR, Proteintech) were used as primary antibodies, incubated overnight at 4 °C.

Techniques: In Vitro, Expressing, Cell Culture, Staining, Co-Culture Assay

Journal: Bioactive Materials

Article Title: Mossy-textured hydroxyapatite-modified poly (lactic-co-glycolic acid) microspheres promote collagen regeneration via calcium/TGF-β and chemokine signaling pathways in soft tissue augmentation

doi: 10.1016/j.bioactmat.2025.12.028

Figure Lengend Snippet: Evaluation of soft tissue filling and inflammatory response in rats. (A, B) Schematic diagram of the soft tissue filling experiment and injection sites, with at least 2 cm spacing between sites. (C) Photographs of subcutaneous soft tissue filling at 2, 4, 8, and 12 weeks. The white circles indicate the soft tissue filling areas. (D) H&E staining of the filled sites. (E, F) Immunofluorescence images and RFI of TNF-α and TGF-β expression at 2 weeks post-filling (n = 6). (G, H) Immunofluorescence images and RFI of CD86 and CD206 expression at 2 weeks post-filling (n = 6). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.

Article Snippet: CD86 polyclonal antibody (1:200, 13395-1-AP, Proteintech), CD206 (1:200, 18704-1-AP, Proteintech), and TNF-α recombinant antibody (1:200, 80258-6-RR, Proteintech) were used as primary antibodies, incubated overnight at 4 °C.

Techniques: Injection, Staining, Immunofluorescence, Expressing

Journal: Brain, Behavior, & Immunity - Health

Article Title: Endotoxin-induced inflammation promotes, via the adrenomedullary system, a hyperglycemic and anti-inflammatory reflex

doi: 10.1016/j.bbih.2026.101183

Figure Lengend Snippet: Glycemic, Corticosterone, and TNF responses after administration of different doses of LPS . A, LPS-induced hyperglycemia is only observable 30 min after administering 100 μg/kg doses. B, Circulating Corticosterone in response to LPS has an earlier onset when higher doses are used. C, Plasmatic TNF response to LPS is proportional to the administered LPS dose and only detectable 50 min after LPS administration. Data are expressed as the mean ± SEM of 7 subjects per group. P values comparing the control group (no LPS) were calculated using repeated measures two-way ANOVA.

Article Snippet: For TNF measurements, MaxiSorp plates were sensitized with rat TNFα mouse recombinant monoclonal antibody (R&D Systems, MAB510R) used as the capture antibody and a goat polyclonal biotinylated antibody (R&D Systems, BAF510) was used as the detection antibody with the signal provided by Streptavidin-biotin (Jackson Immunoresearch, 016-030-084) revealed with TMB and hydrogen peroxide in acetate/citrate buffers.

Techniques: Control

Journal: Brain, Behavior, & Immunity - Health

Article Title: Endotoxin-induced inflammation promotes, via the adrenomedullary system, a hyperglycemic and anti-inflammatory reflex

doi: 10.1016/j.bbih.2026.101183

Figure Lengend Snippet: Indomethacin administration prevents LPS-induced hyperglycemia and enhances the TNF response. A. Indomethacin administration prevents LPS-induced hyperglycemia. B. LPS-induced corticosterone secretion is not modified by prostaglandin inhibition. C. TNF response to LPS is exacerbated by indomethacin. Data are expressed as mean ± SEM of 7 subjects per group. P values comparing the control group (no LPS) were calculated using repeated measures two-way ANOVA in figures A&B and by one-way ANOVA for figure C, where all groups are compared.

Article Snippet: For TNF measurements, MaxiSorp plates were sensitized with rat TNFα mouse recombinant monoclonal antibody (R&D Systems, MAB510R) used as the capture antibody and a goat polyclonal biotinylated antibody (R&D Systems, BAF510) was used as the detection antibody with the signal provided by Streptavidin-biotin (Jackson Immunoresearch, 016-030-084) revealed with TMB and hydrogen peroxide in acetate/citrate buffers.

Techniques: Modification, Inhibition, Control

Journal: Brain, Behavior, & Immunity - Health

Article Title: Endotoxin-induced inflammation promotes, via the adrenomedullary system, a hyperglycemic and anti-inflammatory reflex

doi: 10.1016/j.bbih.2026.101183

Figure Lengend Snippet: β-adrenergic signaling is essential for LPS-induced hyperglycemia and mediates TNF suppression. A. The β-adrenergic antagonist propranolol inhibits LPS-induced glucose secretion; “x2” groups received a second dose of propranolol at minute 30 (indicated by a dark pink arrow), completely preventing the glucose increase. B. Corticosterone in response to LPS alone or in combination with propranolol; “x2” groups received a second dose at 30 min (also indicated by a dark pink arrow). C. TNF response to LPS alone or combined with a single or a double administration of propranolol. Data are expressed as mean ± SEM of 3–5 subjects per group, and P-values comparing the control group (no LPS) were calculated using repeated measures two-way ANOVA. For statistical interpretation, the following symbols were used: α indicates p < 0.05 with respect of group “Propranolol X2”, β indicates p < 0.05 with respect of groups "Propranolol X1 ″, "LPS + propranolol X2″, "Saline" and "Propranolol X2"; a indicates p < 0.05 with respect of groups "Propranolol X1″, "Propranolol X2″, and "Saline". (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: For TNF measurements, MaxiSorp plates were sensitized with rat TNFα mouse recombinant monoclonal antibody (R&D Systems, MAB510R) used as the capture antibody and a goat polyclonal biotinylated antibody (R&D Systems, BAF510) was used as the detection antibody with the signal provided by Streptavidin-biotin (Jackson Immunoresearch, 016-030-084) revealed with TMB and hydrogen peroxide in acetate/citrate buffers.

Techniques: Control, Saline

Journal: Brain, Behavior, & Immunity - Health

Article Title: Endotoxin-induced inflammation promotes, via the adrenomedullary system, a hyperglycemic and anti-inflammatory reflex

doi: 10.1016/j.bbih.2026.101183

Figure Lengend Snippet: The adrenal medulla mediates LPS-induced hyperglycemia, Corticosterone release, and TNF suppression. A. Glycemic responses after i.v. administration of 100 μg/kg of LPS via a remote canula in adrenal-medullectomized (AdMX) animals. B. Circulating corticosterone responses after remote i.v. administration of LPS in AdMX animals. C. Plasmatic TNF levels 50 min after LPS administration are higher in AdMX animals. D. Glycemic response to i.v. infusion of 50 ng/kg/min of adrenaline for 50 min. E. Corticosterone response to an i.v. bolus of 5 μg/kg ACTH. Data are expressed as mean ± SEM of 5 animals, P values were calculated using repeated measures two-way ANOVA ∗ indicates p < 0.05 and ∗∗ indicates p < 0.01 for both "AdMX LPS" and "AdMX Saline" groups for glycemia; + indicates p < 0.05 for "AdMX Saline" and ° p < 0.05 for "AdMX LPS".

Article Snippet: For TNF measurements, MaxiSorp plates were sensitized with rat TNFα mouse recombinant monoclonal antibody (R&D Systems, MAB510R) used as the capture antibody and a goat polyclonal biotinylated antibody (R&D Systems, BAF510) was used as the detection antibody with the signal provided by Streptavidin-biotin (Jackson Immunoresearch, 016-030-084) revealed with TMB and hydrogen peroxide in acetate/citrate buffers.

Techniques: Saline

Journal: Brain, Behavior, & Immunity - Health

Article Title: Endotoxin-induced inflammation promotes, via the adrenomedullary system, a hyperglycemic and anti-inflammatory reflex

doi: 10.1016/j.bbih.2026.101183

Figure Lengend Snippet: Hyperglycemia and TNF suppression are only recovered in AdMX animals that receive adrenaline. A. Glycemic responses after remote i.v. administration of LPS in adrenal-medullectomized (AdMX) animals with continuous infusion of adrenaline or noradrenaline. B. Corticosterone responses after remote i.v. administration of LPS in AdMX animals with constant infusion of adrenaline or noradrenaline. LPS infusion increases corticosterone, but Adrenaline and Noradrenaline cannot increase Corticosterone further. C. Circulating TNF response after remote i.v. administration of LPS in AdMX animals with continuous infusion of adrenaline or noradrenaline. Data are expressed as mean ± SEM of 4 subjects per group. P values were calculated using repeated measures two-way ANOVA for glycemia where ∗∗∗ indicates p < 0.001 with respect to both "AdMX LPS + inf-Sal" and "AdMX LPS + inf-Nadren" groups. One-way ANOVA compared groups with the same LPS doses in TNF and Corticosterone responses.

Article Snippet: For TNF measurements, MaxiSorp plates were sensitized with rat TNFα mouse recombinant monoclonal antibody (R&D Systems, MAB510R) used as the capture antibody and a goat polyclonal biotinylated antibody (R&D Systems, BAF510) was used as the detection antibody with the signal provided by Streptavidin-biotin (Jackson Immunoresearch, 016-030-084) revealed with TMB and hydrogen peroxide in acetate/citrate buffers.

Techniques: